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Klebsiella pneumoniae is an opportunistic pathogen, and its hypervirulent variants cause serious invasive community-acquired infections. A genomic view of K. pneumoniae NTUH-2044 for the carbohydrate phosphotransferase system (PTS) found a putative fructose PTS, namely, the Frw PTS gene cluster. The deletion mutant and the complemented mutant of frwC (KP1_1992), which encodes the putative ...Klebsiella oxytoca (KO) is one of several Klebsiella bacteria. These bacteria are naturally found in the intestinal tract, mouth, and nose. ... Healthline Media does not provide medical advice ...

3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Klebsiella pneumoniae, a ... K. pneumoniae strain M5al was provided by M.J ... Growth media ThemediausedwereLuriabroth ... The evolutionary trade-offs in phage-resistant Klebsiella pneumoniae entail cross-phage sensitization and loss of multidrug resistance. ... as observed by the large deletion in K. pneumoniae 77 plasmid containing bla CTX-M, ant(3 ... Too Short Weak Medium Strong Very Strong Too Long. Password Changed Successfully.Oct 08, 2021 · In this study, we revealed that Gallium nitrate III (GaN) inhibits the growth of a collection of twenty polymyxin-resistant Klebsiella pneumoniae strains at concentrations ranging from 2 to 16µg/mL, using a medium, on which the low iron content and the presence of human serum better mimic the in vivo environment.

The organism, Klebsiella pneumoniae NCTC 418, was cultivated in synthetic medium as described by Evans et aL9 Glycerol was used as the only carbon and energy source. It was assayed enzymatically. Dry weights were determined in duplicates by the method of de Vries and Stouthamer.'' Biomass was collected on a 0.2-pm pore diameter membrane filteraem.asm.orgKlebsiella pneumoniae, Escherichia coli, and other members of the Enterobacteriaceae family are common human pathogens that have acquired broad antibiotic resistance, rendering infection by some strains virtually untreatable.Enterobacteriaceae are intestinal residents, but generally represent <1% of the adult colonic microbiota. Antibiotic-mediated destruction of the microbiota enables ...Figure Legend Snippet: Serum or the serum component transferrin inhibits Ybt-dependent growth. K. pneumoniae growth was measured by optical density at 600 nm (OD 600 ) over 18 h in LB broth iron depleted with addition of 350 µM 2,2′-dipyridyl (DIP) (A), LB alone (B), LB-DIP with 1% (C) or 10% (D) pooled heat-inactivated human serum, LB-DIP ...Roles of β-arrestin and ESBL in Klebsiella pneumoniae growth and ant-microbial drug resistance. In order to assess the role β-arrestin and ESBL in Klebsiella pneumoniae growth, the activities of β-arrestin and ESBL in Klebsiella pneumoniae were first evaluated. As presented in Fig. 2A, the β-arrestin-luciferase signal was higher in AR-K.P. compared with that in NB-K.P.Klebsiella pneumoniae is a well-studied Gram-negative bacteria that has a high growth rate in minimal medium and is already widely used as a microbial factory for the production of 3-hydroxypropionate and 1,3-propanediol (1,3-PDO) . It also has a wide variety of substrates, including glycerol and monosaccharides (glucose, xylose and arabinose ...Klebsiella pneumoniae on MacConkey agar is a mucoid and lactose fermenter isolated from urine as shown above picture. K. pneumoniae s cientific classification is as follows-. Klebsiella is from the surname of German-Swiss microbiologist Edwin Klebs (1834-1913). Klebsiella is a genus of Gram-negative, oxidase-negative, fimbriated, non -motile ....

Glycerol is a byproduct from the soap and detergent industry and possibly from future biodiesel plants. The conversion of glycerol to 1,3-propanediol (PD) is of industrial interest due to the potential use of PD for the synthesis of polyesters. We have been studying the microbial conversion of ...Klebsiella [kleb−see−ell−uh] is a type of Gram-negative bacteria that can cause different types of healthcare-associated infections, including pneumonia, bloodstream infections, wound or surgical site infections, and meningitis. Increasingly, Klebsiella bacteria have developed antimicrobial resistance, most recently to the class of antibiotics known as carbapenems. Growth inhibition in different media. Klebsiella pneumoniae, a member of the family Enterobacteriaceae, is highly versatile metabolically and, as a consequence, is able to grow under a wide variety of conditions.The tellurium compound AS101 inhibited the growth of ESBL-producing and non-ESBL-producing K. pneumoniae isolates in a dose-response manner when the bacteria grew on a rich medium (BH).Results: K. pneumonia cultured at 37°C after 24 h on the meat peptone agar formed large, convex, mucous, and white colonies (d=3.0-6.0 mm). With the growth in the meat and peptone broth, uniform turbidity of the medium was observed. Analyzing the optical density indices (density, D), it was found that K. pneumoniae were good producers of biofilms (D=0.528±0.31).

Images are overlays of fluorescence signal and phase-contrast micrographs. Scale bar, 5 μm. (C) Growth curves of cells grown in C+Y medium at 37°C showing that growth of the modified S. pneumoniae strains JWV500 and MK119 is similar to that of the wild-type strain D39 in vitro . Averages of three replicates are shown.Bacteria can modify their morphology in response to environmental stimuli for survival or host defense evasion. The rich glucose in vivo or in the Luria-Bertani (LB) medium shortened the cell length of Klebsiella pneumoniae. The environmental glucose decreased the levels of cyclic AMP (cAMP) and the transcription of crp, which declined the cAMP-cAMP receptor protein (cAMP-CRP) activity.Oct 08, 2021 · In this study, we revealed that Gallium nitrate III (GaN) inhibits the growth of a collection of twenty polymyxin-resistant Klebsiella pneumoniae strains at concentrations ranging from 2 to 16µg/mL, using a medium, on which the low iron content and the presence of human serum better mimic the in vivo environment. 3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Klebsiella pneumoniae, a ... K. pneumoniae strain M5al was provided by M.J ... Growth media ThemediausedwereLuriabroth ... Klebsiella pneumoniae on MacConkey agar is a mucoid and lactose fermenter isolated from urine as shown above picture. K. pneumoniae s cientific classification is as follows-. Klebsiella is from the surname of German-Swiss microbiologist Edwin Klebs (1834-1913). Klebsiella is a genus of Gram-negative, oxidase-negative, fimbriated, non -motile ...Klebsiella pneumoniae is a Gram-negative, rod-shaped bacillus from the genus Klebsiella and family Enterobacteriaceae ( Boone et al., 2001 ). K. pneumoniae is facultatively anaerobic, oxidase-negative, and produces acid and gas from lactose. It is an enteric bacterium, noted in the intestinal tract of 5% of healthy humans ( Ganaway, 1976 ). four carbapenem-resistant K.pneumoniae strains and the carbapenem-susceptible strain ATCC 13883 using various separation gels and growth media..... 79 Figure 4.3. MALDI-TOF MS analysis of carbapenem-resistant K. pneumoniae Klebsiella pneumoniae, Escherichia coli, and other members of the Enterobacteriaceae family are common human pathogens that have acquired broad antibiotic resistance, rendering infection by some strains virtually untreatable.Enterobacteriaceae are intestinal residents, but generally represent <1% of the adult colonic microbiota. Antibiotic-mediated destruction of the microbiota enables ...

A method for controlling the growth of Klebsiella pneumoniae bacteria in an aqueous system which comprises adding to said system from about 0.1 to 200 parts per weight of a composition per one million parts per weight of said aqueous system, said composition comprising a synergistic mixture of (a) didecyl dimethyl ammonium chloride and (b) 2-(2 ...Klebsiella pneumoniae. Klebsiella pneumoniae is a Gram-negative, non-motile, facultative anaerobe belonging to the Enterobacteriaceae family of the γ-Proteobacteria class in the phylum Proteobacteria.K. pneumoniae consists of straight rods 1 to 2 μm (micrometres) in length with a thick, surrounding capsule ().

Gram-negative bacteria are a significant contributor to disease in many healthcare settings. For example, Gram-negative organisms surpassed Staphylococcus aureus as a major cause of bacteremia at a university medical center and prevalence of Gram-negative isolates continued to rise at that ...Medium Incubation Control Organism Expected Reactions Bile-aesculin agar 24h Enterococcus faecalis Growth and blackening α-Haemolytic Streptococcus No growth, with haemolysis Blood agar 24h, CO 2 Streptococcus pyogenes Growth and β- haemolysis Staphylococcus pneumoniae Growth and α-haemolysis Chocolate agar 24h, CO2 Haemophilus influenzae ...Growth of bacteria was repressed in solid medium containing 1.2 mol/L NaCl. A1501 could grow very slowly even if the NaCl concentration reached 1.6 mol/L in liquid medium. A1501 could produce IAA under salt stress and maintain N-fixing activity until the NaCl concentration was higher than 1.0 mol/LGrowth will occur at an alkaline pH, which turns the media from green too blue, and no growth will result in no change of the media from its green color. Klebsiella pneumoniae is positive for citrate utilization and Escherichia coli is negative for citrate utilization

A culture medium was developed which selectively favored the growth of Klebsiella pneumoniae and Klebsiella oxytoca in Escherichia coli-rich fecal cultures, without the use of antibiotics. The discriminative capacity of this medium was based on the presence of only two carbon sources, citrate and inositol, which can be utilized by nearly all K. pneumoniae and K. oxytoca strains but not by E. coli. Selective media for Klebsiella pneumoniae have been important in studies of hospital-acquired infections. On an agar medium which included ornithine, raffinose, and Koser citrate, K. pneumoniae strains grew as yellow mucoid colonies at 24 h and there was some increase in colony size at 48 h. Other m …Purpose To retrospectively compare the computed tomographic (CT) features of liver abscesses caused by Klebsiella pneumoniae with those caused by other bacterial pathogens. Materials and Methods This retrospective study was approved by the institutional review board, with waiver of informed consent. Hospital records of all patients with a diagnosis of liver abscess between July 2003 and July ...The activated carbon filtration appeared to be the most effective, specific, and rapid method for the selective removal of traces of molybdenum from growth media of N2-fixing bacteria. A new method for the selective removal of traces of molybdenum from growth media of N2-fixing bacteria (Rhodobacter capsulatus and Klebsiella pneumoniae) was developed.A method for controlling the growth of Klebsiella pneumoniae bacteria in an aqueous system which comprises adding to said system from about 0.1 to 200 parts per weight of a composition per one million parts per weight of said aqueous system, said composition comprising a synergistic mixture of (a) didecyl dimethyl ammonium chloride and (b) 2-(2 ...Among Enterobacteriaceae, Klebsiella spp. are recognized mostly in the medical field as famous opportunistic germs associated with pathogenic infections [1,2]. K. pneumoniae represents a saprophytic pathogen that might affect both plants, animals, and humans [1,3,4], but it can be successfully used for biotechnological applications [].In medical terms, K. pneumoniae is a pathogenic strain ...

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  • Klebsiella pneumoniae is a frequent cause of infectious diseases in hospitals and community settings and is associated with a wide variety of clinical conditions including pneumonia, intra-abdominal infections, urinary tract infections, and bloodstream infections (BSIs).K. pneumoniae is reported to be the second most common cause of Gram-negative bacteremia and has a high mortality rate [].
  • Growth of Klebsiella pneumoniae CRE (Carbapenem-resistant enterobacteriaceae) from positive blood culture on MacConkey agar. Photo: Chiara.marraccini/Wikimedia Commons, CC BY 4.0. Found among the top three ranks in WHO's priority list for pathogens , Klebsiella pneumoniae is one of those bacteria against which medical and scientific ...
  • growth in 0.5% glucose increased transcription of orf1, orf3, and orf7 in comparison with growth in 0.1%, or 0.2% glucose (Fig. 2). Table 1. Comparisons of characteristics between diabetic patients with community-acquired K. pneumoniae bacteremia who developed invasive syndrome and those who did not develop invasive syndrome. Invasive syndrome (%)

There is currently no commercial medium to screen gram-negative bacteria harboring the mcr-1 gene. Nordmann et al. described an in-house SuperPolymyxin medium composed of eosin methylene blue agar, 3.5 mg/L of colistin sulfate, 10 mg/L of daptomycin, and 5 mg/L amphotericin B, which showed excellent sensitivity and specificity.Colistin resistance can be confirmed within 2 h by using an in ...

Klebsiella pneumoniae is a respiratory, blood, liver, and bladder pathogen of significant clinical concern. We show that the adaptor protein, SKAP2, is required for protection against K. pneumoniae (ATCC 43816) pulmonary infections.Skap2-/- mice had 100-fold higher bacterial burden when compared to wild-type and burden was controlled by SKAP2 expression in innate immune cells.
Meta Tag: Klebsiella Pneumoniae Symptoms Meta Description: Klebsiella Pneumoniae symptoms include high fever, chills and inflammation and hemorrhage of lungs with destruction of healthy cells. Klebsiella, a kind of bacterium, is found in the intestines, colon, mouth and skin of human beings. This bacterium is found in four out of ten people.
Eleven strains of K. pneumoniae were tested: five were extended spectrum beta lactamase (ESBL)-producing strains and six were non-ESBL-producing strains. The MIC and MBC of ten strains were 9 microg/ml AS101 and 18 microg/ml for one strain. AS101 treatment inhibited bacterial growth in a dose-dependent manner on protein-rich media.
ABSTRACT. In batch mode, Klebsiella pneumoniae growth and 2,3-butanediol/acetoin formation are increasingly inhibited by initial sucrose concentrations (S 0) over 60 g/L.At non inhibitory conditions, a maximum sucrose consumption rate of 1,5 g/L/h was measured. With S 0 =204 g/L however, this rate decreased to 0.15 g/L/h. K. pneumoniae fermented 204 g/L sucrose to produce 84.3 g/L of a mixture ...

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You inoculate klebsiella pneumoniae in your broth and you start measuring the optical density (OD) every half an hour (or 15 min). ... In rich medium like LB growth is likely to slow down and the ...
Klebsiella pneumoniae is an important cause of bloodstream infections in critically-ill patients, with mortality exceeding 50% for infections caused by antibiotic-resistant strains. Despite its importance as a bacteremia-causing agent, there is little known about the metabolism of K. pneumoniae during growth in pure human blood.. Here, we comprehensively profile the volatile metabolites ...

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To evaluate the susceptibility of Klebsiella pneumoniae towards AgNPs, MIC and MBC values were determined. For the evaluation of MICs, broth microdilution assay was used. The results indicated that the MIC value of NPs against Klebsiella pneumoniae MF953599 was 125 μg/ml while for K. pneumoniae MF953600 the MIC value was found to be 62.5 μg/ml.. The data revealed that the MBC value for the ...
Klebsiella pneumoniae, a common gut bacteria, causes problems when it moves outside the gut and causes infection. Learn about its symptoms and treatment.

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Oct 08, 2021 · In this study, we revealed that Gallium nitrate III (GaN) inhibits the growth of a collection of twenty polymyxin-resistant Klebsiella pneumoniae strains at concentrations ranging from 2 to 16µg/mL, using a medium, on which the low iron content and the presence of human serum better mimic the in vivo environment.
A culture medium was developed which selectively favored the growth of Klebsiella pneumoniae and Klebsiella oxytoca in Escherichia coli-rich fecal cultures, without the use of antibiotics. The discriminative capacity of this medium was based on the presence of only two carbon sources, citrate and inositol, which can be utilized by nearly all K. pneumoniae and K. oxytoca strains but not by E. coli.

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The activated carbon filtration appeared to be the most effective, specific, and rapid method for the selective removal of traces of molybdenum from growth media of N2-fixing bacteria. A new method for the selective removal of traces of molybdenum from growth media of N2-fixing bacteria (Rhodobacter capsulatus and Klebsiella pneumoniae) was developed.
Klebsiella pneumoniae, Escherichia coli, and other members of the Enterobacteriaceae family are common human pathogens that have acquired broad antibiotic resistance, rendering infection by some strains virtually untreatable.Enterobacteriaceae are intestinal residents, but generally represent <1% of the adult colonic microbiota. Antibiotic-mediated destruction of the microbiota enables ...

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Djawa photobookKlebsiella pneumoniae is a respiratory, blood, liver, and bladder pathogen of significant clinical concern. We show that the adaptor protein, SKAP2, is required for protection against K. pneumoniae (ATCC 43816) pulmonary infections.Skap2-/- mice had 100-fold higher bacterial burden when compared to wild-type and burden was controlled by SKAP2 expression in innate immune cells.Keywords: Iron, Klebsiella pneumoniae, Growth, Biofilm, Virulence Background Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause both community-acquired and nosocomial infections, in-cluding liver abscesses, pneumonia, urinary tract infec-tions and bacteremia worldwide [1]. In the past twoSelective media for Klebsiella pneumoniae have been important in studies of hospital-acquired infections. On an agar medium which included ornithine, raffinose, and Koser citrate, K. pneumoniae strains grew as yellow mucoid colonies at 24 h and there was some increase in colony size at 48 h. Other m …Primary Klebsiella pneumoniae liver abscess complicated with metastatic meningitis or endophthalmitis is a globally emerging infectious disease. Its pathogenic mechanism remains unclear. The bacterial virulence factors were explored by comparing clinical isolates. Differences in mucoviscosity were observed between strains that caused primary liver abscess (invasive) and those that did not ...

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The plant pathogenic Klebsiella pneumoniae survived in pomegranate orchard soil for more than two years in an infectious stage. The pomegranate seedlings/grafts planted at a site which had the wilt disease plant earlier and left fallow for a season also succumb to Klebsiella infection causing wilt. The bacterium also survives in root bark necrosis lesions for more than two years under ...S. pneumoniae is a fastidious bacterium, growing best at 35-37°C with ~5% CO 2 (or in a candle-jar). It is usually cultured on media that contain blood, but can also grow on a chocolate agar plate (CAP). On a blood agar plate (BAP), colonies of S. pneumoniae

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The use of acridine orange to visualize and quantify spatial variations in growth rate within Klebsiella pneumoniae colonies and biofilm was investigated. Bacterial colonies supported on polycarbonate filter membranes were grown on R2A agar plates.Medication Summary. The following is a discussion on the specific agents used in the antimicrobial therapy of Klebsiella infections. In vitro data show that a wide range of beta-lactams, aminoglycosides, quinolones, and other antibiotics are useful for treatment of klebsiellae infections. [ 17, 18, 19] Cephalosporins have been widely used as ...

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World of darkness character generatorthroughput screening of K. pneumoniae mutants or com-pounds, with a favorable Z0 score (Zhang et al., 1999) (Figure S1B). To determine the genes required for growth in serum, the K. pneumoniae mutant library was arrayed into LB broth and 50% human serum, and growth was measured after 24 h (Table S1).Mar 12, 2015 · aeruginosa, Stenotrophomonas maltophilia and carbapenemase-positive Klebsiella pneumoniae were grown on CHROMagar Acinetobacter® and supplemented SBA media. Susceptible clinical isolates and standard strains of Enterobacteriaceae were inhibited by both CHROMagar Acinetobacter ® and supplemented SBA. C. albicans isolates, inoculated To evaluate the susceptibility of Klebsiella pneumoniae towards AgNPs, MIC and MBC values were determined. For the evaluation of MICs, broth microdilution assay was used. The results indicated that the MIC value of NPs against Klebsiella pneumoniae MF953599 was 125 μg/ml while for K. pneumoniae MF953600 the MIC value was found to be 62.5 μg/ml.. The data revealed that the MBC value for the ...The influence of growth media and media constituents on bacteriocin production byKlebsiella pneumoniae was studied. Among the standard laboratory media used trypticase soy broth (TSB) showed the maximum production and poor yields resulted from growth in peptone water and nutrient broth. A number of peptones differed in their bacteriocin production. Best yields were observed in tryptone and ... Apr 01, 2016 · E.g. the incubated mixtures containing the supernatant of K. pneumoniae UVHC5 and E. coli ATCC 8739 in STI and MRS showed significant differences in particle size after 7 days of incubation compared to the addition of uninoculated culture media, as well as the bacterial growth in MH resulted in significant different particle size determined . Streptococcus pneumoniae (pneumococcus) is a frequent colonizer of the nasopharynx and one of the leading causative agents of otitis media, pneumonia, and meningitis. The current literature asserts that S. pneumoniae is transmitted person to person via respiratory droplets; however, environmental surfaces (fomites) have been linked to the spread of other respiratory pathogens. Desiccation ...The scientists report the mechanism of how natural killer cells, important cells of the innate immune system, control the growth of Klebsiella during lung infection. Klebsiella induces critical immune response regulators, type I interferons (IFNs), which act as middlemen in the crosstalk between alveolar macrophages (immune cells that engulf ...Roon bridge vs roon serverTwitter viral tiktok indonesiaApr 01, 2016 · E.g. the incubated mixtures containing the supernatant of K. pneumoniae UVHC5 and E. coli ATCC 8739 in STI and MRS showed significant differences in particle size after 7 days of incubation compared to the addition of uninoculated culture media, as well as the bacterial growth in MH resulted in significant different particle size determined . Guri iib ah daarusalaampathogen Klebsiella pneumoniae is a Gram-negative bacillus associ-ated with healthcare infections, known to cause variety of diseases in humans with significant morbidity and mortality [7]. K. pneumoniae causes various serious illnesses and the problem is aggravated due to its propensity to acquire multidrug resistance determinants [8,9].Shadow health heent subjective questionsAzula protects zuko fanfictionCulture medium growth [Ref.: #9134] Culture medium link: Medium recipe at BacMedia [Ref.: #9134] Culture medium composition: ... Klebsiella pneumoniae subsp. pneumoniae partial 16S rRNA gene, type strain DSM:30104T, clone K10: HF543831: 1530: ENA. 72407 tax ID: Genome sequence information:!

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  • Otitis media. In cases of otitis media, S. pneumoniae has historically been the most common isolate, being identified in about 40 to 50% of cases in which an etiologic agent is isolated or in 30 to 40% of all cases. Nontypeable H. influenzae, historically the next most common organism, is more likely to predominate in highly vaccinated populations.
  • [0074] Five mM AEP in Nutrient Broth had little effect on the growth of Klebsiella pneumoniae CMCC3077 when compared with the Nutrient Broth control. In contrast alaphosphin was an effective selective agent for the inhibition of Klebsiella pneumoniae CMCC3077 and caused a two to three log reduction in the viable count in Nutrient Broth within a ...
  • You inoculate klebsiella pneumoniae in your broth and you start measuring the optical density (OD) every half an hour (or 15 min). ... In rich medium like LB growth is likely to slow down and the ...
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Klebsiella, (genus Klebsiella), any of a group of rod-shaped bacteria of the family Enterobacteriaceae.Klebsiella organisms are categorized microbiologically as gram-negative, facultative anaerobic, nonmotile bacteria.Klebsiella organisms occur in soil and water and on plants, and some strains are considered a part of the normal flora of the human gastrointestinal tract.A synthetic basal salts medium containing inositol as the sole source for carbon and energy and bromothymol blue as pH indicator, selectively supports the growth of Klebsiella pneumoniae and Serratia spp. On this medium incubated at 37 degrees C for 24 hr, all cultures of Kl. pneumoniae, Serratia liquefaciens and S. marcescens form yellow colonies.

Rinnenhalter trapezblech hornbachAmong Enterobacteriaceae, Klebsiella spp. are recognized mostly in the medical field as famous opportunistic germs associated with pathogenic infections [1,2]. K. pneumoniae represents a saprophytic pathogen that might affect both plants, animals, and humans [1,3,4], but it can be successfully used for biotechnological applications [].In medical terms, K. pneumoniae is a pathogenic strain ...The organism, Klebsiella pneumoniae NCTC 418, was cultivated in synthetic medium as described by Evans et aL9 Glycerol was used as the only carbon and energy source. It was assayed enzymatically. Dry weights were determined in duplicates by the method of de Vries and Stouthamer.'' Biomass was collected on a 0.2-pm pore diameter membrane filter

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Population Genomic Analysis of 1,777 Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae Isolates, Houston, Texas: Unexpected Abundance of Clonal Group 307. mBio , 2017; 8 (3): e00489 ...throughput screening of K. pneumoniae mutants or com-pounds, with a favorable Z0 score (Zhang et al., 1999) (Figure S1B). To determine the genes required for growth in serum, the K. pneumoniae mutant library was arrayed into LB broth and 50% human serum, and growth was measured after 24 h (Table S1)., Undertale multiplayer android.